黄淮海与环渤海设施蔬菜产区重金属累积特征和环境质量评价

黄淮海与环渤海设施蔬菜产区是全国蔬菜发展的六大优势产区之一。通过收集2011～2019年黄淮海与环渤海设施菜田重金属研究的文献数据,对该区域重金属污染和环境质量进行了统计分析。结果表明,黄淮海与环渤海设施蔬菜产区土壤重金属Cu、Zn、Cr、Ni、Pb、Cd、As和Hg的平均含量均低于温室蔬菜产地环境质量评价标准限量值。与各个省市土壤背景值相比,土壤出现了明显的Cu、Zn、Cd和Hg累积,Cr和As均没有出现累积。8种重金属元素单项污染指数范围在0.30～0.80,排序依次为:CdPbHg=NiZnCuAsCr,仅Cd处于警戒等级,其余重金属元素均处于清洁等级。8种重金属综合污染指数平均值为0.75,表明该区域重金属污染处于警戒水平,需采取相应的污染防控措施。     

两种商品木浆的纸浆性能研究

选取市场上具有代表性的两种商品针、阔叶木浆进行PFI打浆处理,研究在不同打浆度下浆料纤维质量、动态滤水性能、Zeta电位、微观结构等方面的变化,并对不同比例配抄条件下的成纸性能进行考察,结果表明:针、阔叶浆的打浆度与打浆转数之间的关系均符合线性分布:y=17.7503+0.0015x,R^2=0.9914(针叶浆);y=8.8986+0.00275x,R^2=0.9734(阔叶浆);经PFI打浆后,两种木浆纤维长度、宽度变化较小,细小组分含量变化明显;随着打浆度的升高,两者的滤水性能下降,Zeta电位先上升然后基本保持不变;初始打浆度下,随着针叶浆比例的减少、阔叶浆比例的增加,成纸的抗张强度先下降再增大,厚度逐渐下降至不变,定量逐渐增加,柔软度逐渐下降;随着打浆度的升高,成纸的抗张强度增大,厚度、定量逐渐下降,针叶浆成纸柔软度逐渐下降,阔叶浆成纸柔软度先升高再逐渐降低。     

Characterization of Spray-Dried Hydrolyzed Proteins from Pink Perch Meat Added with Maltodextrin and Gum Arabic

In the present study, spray-dried pink perch meat protein hydrolysate (PPH) and hydrolysate with maltodextrin and gum arabic (PPHMG) were characterized in relation to their physical, rheological, functional, antioxidant, thermal, and sensory properties. The Herschel–Bulkley model was suitable to explain the flow behavior of PPH and PPHMG emulsions. Both the samples exhibited functional and antioxidant properties. Antioxidant activities were significantly higher for PPH compared with PPHMG (p<0.05). PPH and PPHMG alone did not exhibit antimicrobial activity but enhanced the activity of chitosan against pathogens. Sensory acceptability of the samples in soup revealed that PPHMG of up to 4% was highly scored without any traces of bitterness, whereas for PPH, a concentration of up to 3% was acceptable.     

低温风干工艺下酱鸭加工过程中品质特性变化规律研究

为改善酱鸭的质地、色泽和风味,本试验在传统酱鸭加工工艺的基础上,引入低温风干工艺制备酱鸭,并研究其加工过程中理化特性(水分含量、色泽、剪切力值、质构特性)和挥发性风味成分的变化。结果表明,在加工过程中,酱鸭水分含量从76.55%降至58.27%(P0.05);与原料肉相比,L~*值在风干6 h显著降低,a~*值在腌制和风干结束下降,b~*值和咀嚼性在酱制阶段增加,杀菌阶段下降,硬度在酱制阶段升高,而弹性和内聚性均在杀菌阶段降低;与风干24 h相比,胶着性在杀菌阶段降低(P0.05),剪切力值先升高后下降。酱鸭挥发性风味成分分析显示,酱鸭成品有70种挥发性风味成分,主要为萜烯类、醛类、呋喃类、酮类、烃类和醇类;其中,莰烯、己醛和2-戊基呋喃是酱鸭成品主要的特征风味成分,分别占总相对含量的16.51%、10.44%和11.88%。本试验结果为低温风干工艺下酱鸭品质变化特征的研究提供了一定的理论依据。     

便携式XRF仪现场快速测定土壤中Pb的可行性研究

便携式XRF分析仪具有轻便、快速和可测对象较多与测量元素较广等特点而广泛应用于矿石、土壤等方面快速检测,但土壤的现场快速测定结果的准确性有待进一步研究与探讨。以安宁河谷平原土壤中Pb为研究对象,通过对便携式XRF仪现场快速检测结果与实验室分析结果的对比和影响因素的分析,建立基于安宁河谷平原表层土壤快速测定结果的线性函数为y=1.170 9x-17.326 0(R~2=0.992 9,x为现场检测结果),探讨其影响因素,为该区域表层土壤中Pb快速检测结果提供依据。     

Transgenic sorghum with suppressed synthesis of kafirin subclasses: Effects on flour and dough rheological characteristics

Arising from work showing that conventionally bred high protein digestibility sorghum types have improved flour and dough functionality, the flour and dough properties of transgenic biofortified sorghum lines with increased protein digestibility and high lysine content (TG-HD) resulting from suppressed synthesis of several kafirin subclasses, especially the cysteine-rich γ-kafirin, were studied. TG-HD sorghums had higher flour water solubility at 30 °C (p < 0.05) and much higher paste viscosity (41% higher) than their null controls (NC). TG-HD doughs were twice as strong as their NC and dynamic rheological analysis indicated that the TG doughs were somewhat more elastic up to 90 °C. CLSM of doughs and pastes indicated that TG-HD had a less compact endosperm protein matrix surround the starch compared to their NC. The improved flour and dough functional properties of the TG-HD sorghums seem to be caused by reduced endosperm compactness resulting from suppression of synthesis of several kafirin subclasses which modifies protein body and protein matrix structure, and to improved protein-starch interaction through hydrogen bonding specifically caused by reduction in the level of the hydrophobic γ-kafirin. The improved flour functionality of these transgenic biofortified sorghums can increase their commercial utility by complementing their improved nutritional quality.     

Effects of biodegradable mulch on soil quality

Biodegradable plastic films are desirable alternatives to traditional black polyethylene plastic for use as mulches in agroecosystems. Efforts are ongoing to engineer biodegradable plastic mulches that could be incorporated into the soil at the end of the crop season, and decomposed by microorganisms, ultimately to CO₂, H₂O, and biomass. Whether changes in soil quality occur during or following biodegradation is unknown. An 18-month study evaluated the effects on soil quality following burial of four potentially biodegradable mulches and a no mulch control in high tunnel and open field tomato production systems across three geographically distinct locations (Knoxville, TN; Lubbock, TX; Mount Vernon, WA). The mulch treatments included: two starch-based mulches (BioAgri^® Ag-Film and BioTelo Agri); one experimental 100% polylactic acid mulch (Spunbond-PLA-10); one cellulose-based mulch (WeedGuardPlus; positive control); and a negative control (no mulch). The soil management assessment framework (SMAF) was used to calculate a soil quality index (SQI) according to five dynamic soil properties: microbial biomass carbon, β-glucosidase, electrical conductivity, total organic carbon (TOC), and pH. Within the 18-month evaluation period, the effects of the biodegradable mulches on the SQI were minor, and dependent upon production system and time of incubation at all locations. In general, the SQI was higher in the high tunnel systems for some of the mulch treatments at Knoxville and Lubbock but the opposite was true at Mount Vernon. By the final sampling at 18 months, the SQI was lowest for WeedGuardPlus at Lubbock and Mount Vernon but at Knoxville, the WeedGuardPlus SQI was not significantly different from the no mulch control. Of the five SMAF indicators evaluated, soil microbial biomass and β-glucosidase activity were the most responsive to mulch and production systems, supporting the use of these variables as soil quality indicators for short-term changes due to this agricultural management practice.     

Soil sterilization effects on root growth and formation of rhizosheaths in wheat seedlings

Sterilized soils are frequently used in experiments related to soil biology. Soil sterilization is known to alter physicochemical characteristics of soil, plant growth and community structure of the newly developed bacterial population. However, little information exists regarding soil sterilization effects on belowground processes mediated through root–microbe–soil interactions, e.g., development of rhizosheaths which significantly promote the plant growth under stress environments. The present study was conducted to elucidate effects of soil sterilization on wheat root growth and formation of rhizosheaths in relation to chemical changes caused by soil sterilization and the proportion of expolysaccharide (EPS)-producers in bacterial population recolonizing the sterilized soils. Wheat plants were grown for two weeks under greenhouse conditions either in the unsterilized soil or in soils sterilized by autoclaving (121 °C, 1 h) or by gamma (γ)-irradiation (50 kGy). While soil sterilization had no effect on the release of macronutrients, both sterilization procedures significantly increased the electrical conductivity, water-soluble carbon and DTPA-extractable Mn. Seedlings grown in sterilized soils produced higher root biomass and the rhizosheath soil (RS) mass as compared to those grown in the unsterilized soil. Soil sterilization also increased the root length, surface area, volume and number of tips. In bulk soil, RS and on roots, the proportion of EPS-producers in the total bacterial population was higher in sterilized treatments than in the unsterilized. Amending the unsterilized soil with glucose-C increased the root biomass, whereas adding Mn II increased the RS mass. The results showed that soil sterilization by autoclaving or γ-irradiation increases the root growth and RS mass of wheat seedlings. The water-soluble C and DTPA-extractable Mn released upon sterilization, and the increased proportion of EPS-producers in the bacterial population recolonizing the sterilized soils were involved in the observed effects. The results may have implications in studies using autoclaved or γ-irradiated soils to investigate soil–plant–microbe interactions and signify the need to account for intrinsic stimulatory effects of soil sterilization.     

Cattle manure and inorganic nitrogen fertilizer application effects on soil hydraulic properties and maize yield of two soils of Murewa district,Zimbabwe

This study set out to determine the effects of cattle manure and inorganic N‐fertilizer application on the hydraulic properties and maize yield of a clay and sandy soil in a smallholder farming area of Zimbabwe. Four fields classified as homefields (HF: more fertile and closer to homesteads) and outfields (OF: less fertile and further from the homesteads) were selected on clay and sandy soil. They were subjected to four treatments, control (no fertility amendment), 5, 15 and 25 t/ha cattle manure + 100 kg/ha N (as ammonium nitrate) for 7 years. A two‐way randomized complete block design was used with fertility and field type as the two factors. Clay soil hydraulic properties, which included density of macropores with a diameter >300 μm, unsaturated hydraulic conductivity, steady‐state infiltration rate, moisture retention under low suction and maize grain yield, were significantly improved (P < 0.05) by fertility management compared with the control and were generally comparable between the HF and OF. Fertility management significantly improved maize grain yield on sandy soils but did not enhance hydraulic parameters, thereby indicating poor responsiveness in structural build‐up. On the other hand, significantly different hydraulic parameters between the sandy soil HF and OF suggested effects of other factors not related to soil fertility and field type treatments. We therefore concluded that application of a high rate (25 t/ha) of cattle manure and inorganic N‐fertilizer is beneficial for fertility restoration to the degraded OFs with associated comparable hydraulic properties on HFs and OFs on clay soil, unlike on sandy soil.     

Strong functional stability of soil microbial communities under semiarid Mediterranean conditions and subjected to long-term shifts in baseline precipitation

We investigated the effect of soil microclimate on the structure and functioning of soil microbial communities in a Mediterranean Holm-oak forest subjected to 10 years of partial rain exclusion manipulations, simulating average drought conditions expected in Mediterranean areas for the following decades. We applied a high throughput DNA pyrosequencing technique coupled to parallel measurements of microbial respiration (R_H) and temperature sensitivity of microbial respiration (Q₁₀). Some consistent changes in the structure of bacterial communities suggest a slow process of community shifts parallel to the trend towards oligotrophy in response to long-term droughts. However, the structure of bacterial communities was mainly determined by short-term environmental fluctuations associated with sampling date (winter, spring and summer) rather than long-term (10 years) shifts in baseline precipitation. Moreover, long-term drought did not exert any chronic effect on the functioning of soil microbial communities (R_H and Q₁₀), emphasizing the functional stability of these communities to this long-term but mild shifts in water availability. We hypothesize that the particular conditions of the Mediterranean climate with strong seasonal shifts in both temperature and soil water availability but also characterized by very extreme environmental conditions during summer, was acting as a strong force in community assembling, selecting phenotypes adapted to the semiarid conditions characterizing Mediterranean ecosystems. Relations of climate with the phylogenetic structure and overall diversity of the communities as well as the distribution of the individual responses of different lineages (genera) to climate confirmed our hypotheses, evidencing communities dominated by thermotolerant and drought-tolerant phenotypes.